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6S RNA as a tool to increase production of antibiotics in Streptomyces coelicolor
Title statement 6S RNA as a tool to increase production of antibiotics in Streptomyces coelicolor / Inesa Musić Additional Variant Titles 6S RNA jako nástroj pro navýšení produkce antibiotik u Streptomyces coelicolor Personal name Musić, Inesa, (dissertant) Phys.des. 76 listů : ilustrace, grafy Note Oponent Oldřich Benada. Vedoucí práce Jan Bobek. Název a text práce v anglickém jazyce Abstract This diploma thesis focuses on the phenotypic effect of 6S RNA in model organism Streptomyces coelicolor. 6S RNA is small non-protein-coding RNA. As it was shown in Escherichia coli, 6S RNA imitates structure of DNA promoter and traps sigma70-RNA polymerase, consequently blocking further transcription. Recently, 6S-like RNA was also discovered in Streptomyces (designated here as 6SSc for clarity), but its effect on their complex life cycle remains unknown. Here, we tested whether 6SSc co-regulates transition from the vegetative growth to the stationary phase. When 6SSc RNA was overexpressed in S. coelicolor, this transition was reflected by limited substrate mycelium growth hyper-sporulation, and an over-production of secondary metabolites, actinorhodin and undecylprodigiosin, whose production was measured spectrophotometrically. Hence, we hypothesize that this RNA regulator may also affect the production of other bioactive substances in Streptomyces, including clinically relevant antibiotics. This diploma thesis focuses on the phenotypic effect of 6S RNA in model organism Streptomyces coelicolor. 6S RNA is small non-protein-coding RNA. As it was shown in Escherichia coli, 6S RNA imitates structure of DNA promoter and traps sigma70-RNA polymerase, consequently blocking further transcription. Recently, 6S-like RNA was also discovered in Streptomyces (designated here as 6SSc for clarity), but its effect on their complex life cycle remains unknown. Here, we tested whether 6SSc co-regulates transition from the vegetative growth to the stationary phase. When 6SSc RNA was overexpressed in S. coelicolor, this transition was reflected by limited substrate mycelium growth hyper-sporulation, and an over-production of secondary metabolites, actinorhodin and undecylprodigiosin, whose production was measured spectrophotometrically. Hence, we hypothesize that this RNA regulator may also affect the production of other bioactive substances in Streptomyces, including clinically relevant antibiotics. Another responsib. Benada, Oldřich (opponent)
Bobek, Jan (thesis advisor)Another responsib. Univerzita J.E. Purkyně v Ústí nad Labem. Katedra biologie (degree grantor) Subj. Headings experimentální biologie * Streptomyces Subj. Headings Streptomyces coelicolor * actinorhodin * undecylprodigiosin * secondary metabolism Form, Genre diplomové práce Conspect 57 - Obecná biologie UDC 57.08 * 579.873.7 * (043)378.2 Country Česko Language angličtina URL https://portal.ujep.cz/StagPortletsJSR168/CleanUrl?urlid=prohlizeni-prace-detail&praceIdno=00178957 Document kind Diploma theses 
Call number Barcode Sublocation Volný výběr Info DA 9768 3300709768 sklad C In-Library Use Only
Number of the records: 1