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6S RNA as a tool to increase production of antibiotics in Streptomyces coelicolor
Údaje o názvu 6S RNA as a tool to increase production of antibiotics in Streptomyces coelicolor / Inesa Musić Další variantní názvy 6S RNA jako nástroj pro navýšení produkce antibiotik u Streptomyces coelicolor Osobní jméno Musić, Inesa, (autor diplomové práce nebo disertace) Fyz.popis 76 listů : ilustrace, grafy Poznámky Oponent Oldřich Benada. Vedoucí práce Jan Bobek. Název a text práce v anglickém jazyce Abstrakt This diploma thesis focuses on the phenotypic effect of 6S RNA in model organism Streptomyces coelicolor. 6S RNA is small non-protein-coding RNA. As it was shown in Escherichia coli, 6S RNA imitates structure of DNA promoter and traps sigma70-RNA polymerase, consequently blocking further transcription. Recently, 6S-like RNA was also discovered in Streptomyces (designated here as 6SSc for clarity), but its effect on their complex life cycle remains unknown. Here, we tested whether 6SSc co-regulates transition from the vegetative growth to the stationary phase. When 6SSc RNA was overexpressed in S. coelicolor, this transition was reflected by limited substrate mycelium growth hyper-sporulation, and an over-production of secondary metabolites, actinorhodin and undecylprodigiosin, whose production was measured spectrophotometrically. Hence, we hypothesize that this RNA regulator may also affect the production of other bioactive substances in Streptomyces, including clinically relevant antibiotics. This diploma thesis focuses on the phenotypic effect of 6S RNA in model organism Streptomyces coelicolor. 6S RNA is small non-protein-coding RNA. As it was shown in Escherichia coli, 6S RNA imitates structure of DNA promoter and traps sigma70-RNA polymerase, consequently blocking further transcription. Recently, 6S-like RNA was also discovered in Streptomyces (designated here as 6SSc for clarity), but its effect on their complex life cycle remains unknown. Here, we tested whether 6SSc co-regulates transition from the vegetative growth to the stationary phase. When 6SSc RNA was overexpressed in S. coelicolor, this transition was reflected by limited substrate mycelium growth hyper-sporulation, and an over-production of secondary metabolites, actinorhodin and undecylprodigiosin, whose production was measured spectrophotometrically. Hence, we hypothesize that this RNA regulator may also affect the production of other bioactive substances in Streptomyces, including clinically relevant antibiotics. Dal.odpovědnost Benada, Oldřich (oponent)
Bobek, Jan (vedoucí diplomové práce nebo disertace)Dal.odpovědnost Univerzita J.E. Purkyně v Ústí nad Labem. Katedra biologie (udělovatel akademické hodnosti) Předmět.hesla experimentální biologie * Streptomyces Předmět.hesla Streptomyces coelicolor * actinorhodin * undecylprodigiosin * secondary metabolism Forma, žánr diplomové práce Konspekt 57 - Obecná biologie MDT 57.08 * 579.873.7 * (043)378.2 Země vyd. Česko Jazyk dok. angličtina URL https://portal.ujep.cz/StagPortletsJSR168/CleanUrl?urlid=prohlizeni-prace-detail&praceIdno=00178957 Druh dok. Vysokoškolské práce 
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